TABLE 2.
Oligonucleotides used in this study
| Primer | Sequence (5′ to 3′) | Description |
|---|---|---|
| Erm 1 | GGCGCGCCCGGGCCCAAAATTTGTTTGAT | ermAM cassette |
| Erm2 | GGCCGGCCAGTCGGCAGCGACTCATAGAAT | ermAM cassette |
| CitM1 | TCTGTTGTAGGAGTGATGGGGG | citM mutagenesis |
| CitM2a | GGCGCGCCCTCAGTGCCGTAAAAGGCGAC | citM mutagenesis |
| CitM3b | GGCCGGCCCTTAACGGGCTTAGACATGGGC | citM mutagenesis |
| CitM4 | TCGGTAACGATCTCTCGTAGGC | citM mutagenesis |
| OAD1 | CAATCTGAATGGGAGCCTATTTCG | oad mutagenesis |
| OAD2a | GGCGCGCCCCGCCGTTAATAACCTGAGTTAGGAC | oad mutagenesis |
| OAD3b | GGCCGGCCCGCAGTAGGTGCTAATGTTTCAGGAC | oad mutagenesis |
| OAD4 | TGACCATCAGGATAACGGCG | oad mutagenesis |
| CLY1 | TTCTGTTATCGCAGGTGGCTTAC | cly mutagenesis |
| CLY2a | GGCGCGCCCAAGGAGCATCTCGCAGCATAGC | cly mutagenesis |
| CLY3b | GGCCGGCCCCGCTATTGTTGGTAATCCTCAACC | cly mutagenesis |
| CLY4 | TGTCCGCCAGCTTCCTTAGTAG | cly mutagenesis |
| ASPB1 | TGCTGGATGAAGCGAAACTTACTC | aspB mutagenesis |
| ASPB2a | GGCGCGCCAGGTGTTACAAAATCAGGCTGTCC | aspB mutagenesis |
| ASPB3b | GGCCGGCCCAGGTGTCGCTTTGGTAACAGG | aspB mutagenesis |
| ASPB4 | GCATTGCCCGATAAGATTGGAC | aspB mutagenesis |
| CitMRT1 | AGTTGTTATCCTTGTCTTCGCTGC | citM real-time PCR |
| CitMRT2 | CGATGACTAAGCCCCAGAAACC | citM real-time PCR |
a
Underlined bases indicate the AscI restriction sequence.
b
Underlined bases indicate the FseI restriction sequence.