FIG. 5.

Acetic acid or acetate uptake in ISO membrane vesicles of A. aceti IFO 3283. The transport of [1-¹⁴C]acetate was measured with ISO membrane vesicles at 537 μg (A) or 540 μg (B) of protein in 50 mM KPB (pH 6.5) containing 5 mM MgSO₄, as described in Materials and Methods. When indicated, 0.025 μM nigericin (Nig), 1 μM valinomycin (Val), 10 μM CCCP, or 100 μM DCCD was added to the ISO membrane vesicles suspension and incubated before the addition of [1-¹⁴C]acetate. (A) The reaction was carried out in the absence (•) or presence of 10 mM lactate (□), lactate and Nig (▴), lactate and Val (○), or lactate, Nig and Val (▵). (B) The reaction was done in the absence (•) or presence of 3 mM ATP (□), ATP and CCCP (○), or ATP and DCCD (▵).