FIG. 4.

A. Determination of acetate in the culture supernatant. After the indicated time intervals, supernatants of the WT (▪), PBM001 (•), MGM001 (▴), MB290 (▾), and MB288 (♦), cultivated in BHI medium, were analyzed for acetate concentration as described in Materials and Methods. Shown are representative results of at least two independent experiments. B. Expression of acetyl-CoA synthetase (acsA) in the WT, PBM001, and MB290. Gene expression of acsA was determined in the WT, PBM001 (clpC mutant), and MB290 (sigB mutant) by real-time RT-PCR at different time intervals as described in the legend to Fig. 3.