FIG. 6.

Immunoblot analysis of FRD1200 expressing mutant AlgG proteins. Lanes: (1) wild-type AlgG from plasmid pMF55; (2) P325A proposed catalytic domain mutation; (3) D327A proposed catalytic domain mutation; (4) R316; (5) N362 mutation, recessive to chromosomally encoded S272N of FRD462; (6) pMF54 plasmid lacking algG; (7) D317A; (8) Y321F; (9) N362A; (10) N367A mutation that likely disrupts proper protein folding; (11) V383A; (12) D336A mutation that has no effect on alginate epimerization or secretion.