FIG. 3.

RT-PCR (A) and Northern blot (B) analyses of omcF expression. Total RNA was extracted from acetate-fumarate or acetate-Fe(III) citrate-grown wild-type cultures. (A) The following primers were used for cDNA synthesis: lane 2, none; lane 3, omcF-specific primers RT4017F and RT4017R (Table 1); lane 4, random hexamers; lane 5, random hexamers. (B) The Northern blot was hybridized with ³²P-labeled probes specific for omcF as indicated. Equal amounts of mRNA (20 μg/lane) were loaded. (C) Ethidium bromide-stained gel showing the positions of 16S and 23S rRNA, as a loading control.