FIG. 6.

Northern blot analysis of omcB and omcC expression. Total RNA was extracted from acetate-fumarate- or acetate-Fe(III) citrate-grown cultures of wild-type, OmcF-deficient (omcF::kan), and complemented (omcF::kan/pRG5-omcF) strains and hybridized with ³²Plabeled probes for omcB (A) and omcC (B). Equal amounts of mRNA (5 μg/lane) were loaded for each strain, and RNA quantification was confirmed by ethidium bromide staining (C). The probes were specific for bp 508 to 1004 of omcB and bp 285 to 897 of omcC.