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Purification of C. vibrioforme GTR. GTR was expressed from the pQE30 vector and was purified by Ni-NTA agarose column chromatography under native conditions. Proteins were separated by SDS-PAGE and visualized by Coomassie blue staining. Lane 1, cell lysate; lane 2, flowthrough from the Ni-NTA agarose column; lane 3, washthrough; lane 4, eluate containing GTR. The arrow indicates the position of GTR at approximately 49 kDa.
