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(A) Gel filtration of expressed recombinant C. vibrioforme GTR through Superdex 300 HR 10/30 in the absence and presence of 10% (vol/vol) glycerol. (B) Standard calibration curve for the column using proteins having known molecular weights. The open circles indicate the elution positions of purified C. vibrioforme GTR at different concentrations of glycerol.
