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Polyacrylamide gels stained for heme-associated peroxidase activity with TMBZ-H2O2 (A) and for protein with Coomassie blue (B). Before protein samples were loaded onto the gel, they were denatured by boiling either in the presence of 100 mM DTT (lane −) or in the presence of 100 mM DTT plus 2.5% (vol/vol) β-mercaptoethanol (lane +).
