TABLE 2.

Statistical analyses of Bacteria and Archaea 16S rRNA clone libraries using standard ecological and molecular estimates of sequence diversity

Domain	Nucleic acid sampled	Sample layer	No. of clones screened	No. of operational taxonomic units	% Coverage	Species richness	Sorensen's index	Shannon- Weiner index	Gene diversity	Nucleotide diversity	θ(π)
Bacteria	DNA	SEH	34	35	42.9	69 (57, 81)a		2.785	0.95 ± 0.02b	0.22 ± 0.11b	85.0 ± 41.7b
		IH	46	26	64.0	45 (38, 53)		2.762	0.93 ± 0.02	0.24 ± 0.12	97.5 ± 47.4
		Total	80	55	60.6	77 (71, 82)	0.238	3.168	0.95 ± 0.01	0.23 ± 0.11	96.0 ± 46.2
	RNA	SEH	48	18	81.3	37 (14, 60)		2.591	0.93 ± 0.02	0.17 ± 0.08	82.0 ± 39.9
		IH	49	22	75.5	39 (30, 47)		2.811	0.94 ± 0.02	0.17 ± 0.08	76.8 ± 37.3
		Total	97	30	83.5	50 (44, 57)	0.500	2.946	0.94 ± 0.01	0.17 ± 0.08	83.0 ± 39.9
Archaea	DNA	SEH	50	12	82.0	33 (22, 45)		1.675	0.72 ± 0.05	0.08 ± 0.04	23.3 ± 11.6
		IH	50	14	92.0	15 (14, 16)		2.375	0.91 ± 0.02	0.21 ± 0.10	58.9 ± 28.7
		Total	100	20	94.0	24 (22, 27)	0.519	2.527	0.90 ± 0.02	0.17 ± 0.08	46.4 ± 22.5
	RNA	SEH	49	6	95.9	7 (4, 10)		1.301	0.69 ± 0.04	0.08 ± 0.04	44.7 ± 21.9
		IH	48	4	97.9	4 (4, 4)		0.897	0.49 ± 0.07	0.04 ± 0.02	23.3 ± 11.6
		Total	97	7	96.9	9 (4, 14)	0.600	1.293	0.69 ± 0.02	0.07 ± 0.03	38.3 ± 18.6

^aThe numbers in parentheses are 95% confidence intervals.

^bMean ± standard deviation.