FIG. 1.
Effect of temperature, oxygen concentration, and puromycin on growth of the C. jejuni htrA mutant on solid surfaces. Serial dilutions (10−1, 10−2, 10−3, 10−4, 10−5, and 10−6) of C. jejuni NCTC11168 (wt) and LB1281 (htrA) cultures with an OD600 of 0.1 were spotted in 10-μl volumes onto base II agar plates. The plates were incubated at 37, 42, or 44°C under microaerobic conditions (A), at 37°C under microaerobic conditions on a base II agar plate containing 5 μg/ml puromycin (B), or at 42°C in a candle jar providing 17 to 18% oxygen (C). One representative of three independent experiments is presented.