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Complementation of an E. coli htrA mutant. E. coli strains (wt or htrA21 mutant) containing pLB262 (C. jejuni htrA) or pCR2.1 (vector) were streaked on Luria-Bertani agar plates containing kanamycin (50 μg/ml) and incubated at 30°C (A), 39°C (B), or 42°C (C) overnight.
