FIG. 3.
Effect of the htrA mutation on protein composition of C. jejuni. Proteins were extracted from C. jejuni NCTC11168 (wt) and LB1281 (htrA) cells growing in exponential phase (OD600 = 0.3) at 37 or 42°C. (A) Extracted proteins were separated on a SDS-polyacrylamide gel electrophoresis gel, and the 100-kDa band (arrow) was cut out and identified by mass spectrometry as ClpB. Lane M, molecular weight markers. (B) In a Western blot, proteins were transferred to a PVDF membrane and probed with rabbit polyclonal anti-DnaK (E. coli). An arrow indicates the DnaK band.