TABLE 3.

Production of naringenin, pinocembrin, phloretin, and 2′,4′,6′-trihydroxydihydrochalcone by S. cerevisiae AH22 coexpressing PAL, 4CL, and CHS

Product	Amt in supernatant (mg liter−1)		Amt in cell extract (μg g−1 CDW)		Final total concentration (mg liter−1)
	MeOHa	MeOH/HClb	MeOHc	MeOH/HCld
Naringenin	5.8 ± 0.3	5.4 ± 0.4	140 ± 10	180 ± 30e	∼7
Pinocembrin	ND	NDf	90 ± 10	110 ± 20	∼0.8
Phloretin	7.1 ± 0.4	7.2 ± 0.3	200 ± 10	300 ± 40g	∼9
2′,4′,6′-Trihydroxydihydrochalcone	5.2 ± 0.3	4.4 ± 0.1	810 ± 60	970 ± 130	∼11

^aThe supernatant of the yeast culture was mixed with an equal volume of methanol before HPLC injection.

^bThe supernatant of the yeast culture (5 ml) was freeze-dried and treated with a methanol-HCl solution.

^cThe cell pellet (from 5 ml of yeast culture) was lyophilized and extracted with 70% methanol.

^dThe cell pellet (from 5 ml of yeast culture) was lyophilized and extracted with a methanol-HCl solution.

^eP < 0.1.

^fND, not detectable.

^gP < 0.05.