TABLE 1.
List of bacterial strains and plasmids
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | Strain with high-efficiency cloning, enables α-complementation | Bethesda Research Laboratories |
| ABLE C | Cloning strain, reduces copy number of ColE1-based vectors by fourfold | Strategene, La Jolla, CA |
| L. helveticus CNRZ32 | Wild type | Steele Laboratory collection |
| L. lactis LM0230 | Plasmid-free laboratory strain | 13 |
| Plasmids | ||
| pTRKH2 | EmrlacZ; 6.9 kb | 30 |
| pJDC9 | EmrlacZ; 6.85 kb | 4 |
| pTRKH-N | 3.8-kb SmaI-SalI fragment containing L. helveticus CNRZ32 pepN ligated into pTRKH2; Emr | J. E. Christensen (Steele Laboratory collection) |
| pSUWL29 | 3.0-kb PstI fragment containing CNRZ32 pepO2 ligated into pJDC9 | Y.-S. Chen (Steele Laboratory collection) |
| pSUW650 | 2.6-kb BamHI-KpnI fragment containing pepO3 ligated into pJDC9 | This study |
| pSUW651 | 2.5-kb BamHI-KpnI fragment containing pepF ligated into pJDC9 | This study |
| pSUW652 | 2.1-kb BamHI-KpnI fragment containing pepE2 ligated into pJDC9 | This study |
| pSUW653 | 2.0-kb SmaI-XbaI fragment containing pepE ligated into pJDC9 | This study |
| pSUW660 | 0.2-kb KpnI-BamHI fragment containing CNRZ32 PpepO3 ligated into pBlueskript II SK (+) | This study |
| pSUW661 | 2.4-kb BamHI-XbaI ORF containing CNRZ32 pepO2 ligated into pSUW660 | This study |
| pSUW662 | 1.7-kb BamHI-XbaI ORF containing CNRZ32 pepE ligated into pSUW660 | This study |
| pSUW663 | 2.5-kb BamHI-SacI ORF containing CNRZ32 pepF ligated into pSUW660 | This study |
| pSUW664 | 2.6-kb PCR fragment containing CNRZ32 pepO3 from pSUW650 ligated into pTRKH2 | This study |
| pSUW665 | 2.6-kb PvuII-XbaI fragment containing CNRZ32 pepO2 from pSUW661 ligated into pTRKH2 | This study |
| pSUW666 | 1.9-kb PvuII-XbaI fragment containing CNRZ32 pepE from pSUW662 ligated into pTRKH2 | This study |