TABLE 2.
Quantitative PCR analysis by TaqMan assay for representation of 10 loci in 84 different MDA reactions
| MDA no. | % Representation of indicated locus
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Exo | OmpA | GlyS | TopA | HolA | CadA | ExuR | PcnB | EutC | Nth | |
| 1 | 1.4 | 0.0 | 0.1 | 0.0 | 14.5 | 0.3 | 0.0 | 0.2 | 0.0 | 2.3 |
| 2 | 0.3 | 0.0 | 6.5 | 1.7 | 0.6 | 3.6 | 7.6 | 0.0 | 0.9 | 0.4 |
| 3 | 139.0 | 95.3 | 0.0 | 0.0 | 4.0 | 0.5 | 0.0 | 0.0 | 10.7 | 0.5 |
| 4 | 0.0 | 52.4 | 0.3 | 0.2 | 0.4 | 0.0 | 0.0 | 0.0 | 0.7 | 24.7 |
| 5 | 62.1 | 102.1 | 0.0 | 0.3 | 0.0 | 20.8 | 15.4 | 0.0 | 0.1 | 0.1 |
| 6 | 130.2 | 0.6 | 0.0 | 0.1 | 4.6 | 0.0 | 0.2 | 12.2 | 2.1 | 1.1 |
| 7 | 45.4 | 0.0 | 0.0 | 0.0 | 0.0 | 2.1 | 0.1 | 0.0 | 0.0 | 0.0 |
| 8 | 0.0 | 11.2 | 28.6 | 0.0 | 2.8 | 0.0 | 0.1 | 0.0 | 0.2 | 0.1 |
| 9 | 0.7 | 77.1 | 20.7 | 230.5 | 50.0 | 8.8 | 12.3 | 1.0 | 12.6 | 74.2 |
| 10 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 11 | 55.3 | 214.8 | 29.4 | 56.8 | 17.4 | 20.5 | 8.5 | 35.7 | 52.2 | 58.6 |
| 12 | 119.7 | 90.0 | 31.9 | 55.5 | 15.4 | 22.2 | 23.4 | 14.3 | 1.6 | 65.1 |
| 13 | 41.5 | 260.9 | 17.9 | 4.2 | 0.1 | 0.0 | 5.5 | 0.0 | 39.0 | 13.8 |
| 14 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 15 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 16 | 2.4 | 0.9 | 0.0 | 9.4 | 737.0 | 0.0 | 140.7 | 1.2 | 19.6 | 0.0 |
| 17 | 100.6 | 0.0 | 0.0 | 0.4 | 0.0 | 2.8 | 0.2 | 0.0 | 0.0 | 0.0 |
| 18 | 93.9 | 6.3 | 0.0 | 0.0 | 0.0 | 169.0 | 6.0 | 0.4 | 0.0 | 0.0 |
| 19 | 0.1 | 0.0 | 0.0 | 0.0 | 0.4 | 0.0 | 0.1 | 0.0 | 0.0 | 0.0 |
| 20 | 4.5 | 0.0 | 54.6 | 59.1 | 1.3 | 67.2 | 127.7 | 2.4 | 31.5 | 2.6 |
| 21 | 226.4 | 1.0 | 0.0 | 67.1 | 100.0 | 0.0 | 28.7 | 14.2 | 74.6 | 0.6 |
| 22 | 8.4 | 178.7 | 0.9 | 146.4 | 293.5 | 0.6 | 1.8 | 8.7 | 9.6 | 2.3 |
| 23 | 0.0 | 0.5 | 1.6 | 214.0 | 1.9 | 26.5 | 0.1 | 14.9 | 0.4 | 0.0 |
| 24 | 14.8 | 3.0 | 136.0 | 12.7 | 11.7 | 18.9 | 2.7 | 0.9 | 0.4 | 47.5 |
| 25 | 7.7 | 63.2 | 167.8 | 141.2 | 14.2 | 0.4 | 17.3 | 5.2 | 0.0 | 44.6 |
| 26 | 1.3 | 7.2 | 0.0 | 0.0 | 0.0 | 26.9 | 14.9 | 257.3 | 0.0 | 1.8 |
| 27 | 60.9 | 23.5 | 24.7 | 39.7 | 16.6 | 49.4 | 24.1 | 39.8 | 16.2 | 4.2 |
| 28 | 0.0 | 0.0 | 53.7 | 0.0 | 0.0 | 11.4 | 0.2 | 0.0 | 1.3 | 0.0 |
| 29 | 84.5 | 24.1 | 33.3 | 1.9 | 302.0 | 0.6 | 0.0 | 1.6 | 0.9 | 0.0 |
| 30 | 19.5 | 0.0 | 0.0 | 0.6 | 0.0 | 26.8 | 76.7 | 11.3 | 0.0 | 266.1 |
| 31 | 49.2 | 1.2 | 58.6 | 14.3 | 71.2 | 18.8 | 135.4 | 6.4 | 104.5 | 10.8 |
| 32 | 0.0 | 8.7 | 143.2 | 0.0 | 0.0 | 5.6 | 4.7 | 0.0 | 6.0 | 0.1 |
| 33 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 34 | 21.1 | 0.0 | 195.9 | 0.4 | 20.1 | 0.0 | 84.4 | 6.1 | 0.1 | 5.1 |
| 35 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 36 | 48.2 | 82.7 | 0.9 | 109.1 | 50.9 | 1.9 | 0.3 | 2.0 | 4.4 | 265.6 |
| 37 | 0.0 | 0.0 | 0.0 | 0.1 | 0.0 | 0.0 | 569.3 | 0.0 | 0.0 | 1.6 |
| 38 | 32.3 | 56.2 | 6.9 | 4.4 | 569.4 | 27.6 | 150.5 | 0.6 | 4.1 | 0.8 |
| 39 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 40 | 16.4 | 79.6 | 86.5 | 25.8 | 39.5 | 49.2 | 0.0 | 16.9 | 30.6 | 20.8 |
| 41 | 29.4 | 22.3 | 43.9 | 12.8 | 4.1 | 0.7 | 6.8 | 0.4 | 0.1 | 0.7 |
| 42 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 12.6 | 128.5 |
| 43 | 98.1 | 25.3 | 0.0 | 0.1 | 25.0 | 30.3 | 7.2 | 21.1 | 0.6 | 10.4 |
| 44 | 0.1 | 22.4 | 82.4 | 88.6 | 1.6 | 0.7 | 0.0 | 0.7 | 55.2 | 177.0 |
| 45 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 46 | 0.0 | 0.0 | 37.9 | 0.1 | 138.5 | 0.0 | 445.9 | 0.0 | 0.1 | 0.0 |
| 47 | 0.0 | 24.0 | 0.0 | 39.2 | 5.8 | 0.2 | 0.5 | 0.0 | 0.5 | 1.0 |
| 48 | 1.6 | 0.3 | 28.7 | 1.1 | 305.2 | 87.3 | 0.0 | 11.1 | 89.3 | 38.8 |
| 49 | 11.3 | 0.2 | 50.1 | 40.9 | 14.0 | 61.4 | 3.8 | 93.4 | 0.0 | 260.8 |
| 50 | 0.2 | 17.7 | 8.5 | 14.6 | 0.1 | 0.0 | 21.9 | 15.4 | 4.1 | 0.3 |
| 51 | 90.1 | 0.1 | 0.0 | 0.0 | 0.0 | 207.2 | 0.0 | 0.0 | 0.0 | 0.0 |
| 52 | 16.0 | 6.0 | 7.5 | 0.5 | 107.9 | 11.2 | 25.6 | 3.3 | 88.2 | 272.7 |
| 53 | 33.2 | 47.6 | 20.3 | 104.9 | 91.1 | 0.3 | 14.7 | 32.4 | 7.4 | 252.9 |
| 54 | 10.4 | 24.8 | 9.7 | 5.9 | 0.0 | 0.9 | 112.5 | 0.1 | 0.6 | 59.2 |
| 55 | 0.0 | 0.0 | 13.6 | 0.1 | 0.0 | 0.0 | 0.0 | 126.8 | 0.0 | 0.0 |
| 56 | 15.8 | 512.6 | 7.4 | 20.1 | 0.0 | 19.2 | 299.0 | 0.0 | 1.0 | 946.5 |
| 57 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 58 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 59 | 0.0 | 0.0 | 0.4 | 25.2 | 0.0 | 0.0 | 0.2 | 0.0 | 7.1 | 0.0 |
| 60 | 25.2 | 97.1 | 12.0 | 86.2 | 184.3 | 4.1 | 51.1 | 21.9 | 1.6 | 0.8 |
| 61 | 67.6 | 5.3 | 0.0 | 0.0 | 276.9 | 23.0 | 0.0 | 0.5 | 11.4 | 86.1 |
| 62 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 63 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 64 | 3.2 | 0.0 | 83.9 | 9.4 | 1.2 | 137.0 | 3.4 | 14.2 | 2.1 | 19.9 |
| 65 | 5.2 | 219.8 | 49.0 | 18.2 | 82.7 | 27.7 | 8.7 | 173.2 | 144.1 | 0.0 |
| 66 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 67 | 3.0 | 6.9 | 0.6 | 6.0 | 0.0 | 0.0 | 0.9 | 0.8 | 736.4 | 1.1 |
| 68 | 73.3 | 304.5 | 12.8 | 41.9 | 21.4 | 0.0 | 28.9 | 0.8 | 16.8 | 65.7 |
| 69 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 70 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 71 | 0.6 | 164.8 | 2.8 | 0.0 | 41.6 | 2.2 | 9.9 | 2.6 | 0.6 | 0.0 |
| 72 | 0.1 | 1,211.1 | 68.9 | 3.6 | 103.0 | 2.7 | 35.5 | 111.2 | 0.0 | 0.1 |
| 73 | 0.1 | 67.5 | 50.9 | 53.5 | 24.2 | 2.3 | 0.4 | 18.6 | 0.0 | 236.5 |
| 74 | 0.0 | 11.3 | 0.0 | 68.1 | 52.5 | 19.7 | 0.0 | 11.0 | 0.0 | 187.7 |
| 75 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 76 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 77 | 175.6 | 6.2 | 24.2 | 26.1 | 233.7 | 11.7 | 37.9 | 7.5 | 22.1 | 23.9 |
| 78 | 209.0 | 0.0 | 158.9 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 4.4 | 0.0 |
| 79 | 14.5 | 8.4 | 0.0 | 90.0 | 0.0 | 0.0 | 22.0 | 3.0 | 0.4 | 82.8 |
| 80 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 81 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 | 0.0 |
| 82 | 0.1 | 141.5 | 0.1 | 463.4 | 0.0 | 74.1 | 0.4 | 0.0 | 0.0 | 0.1 |
| 83 | 0.0 | 152.8 | 0.0 | 0.0 | 0.0 | 8.0 | 0.2 | 0.0 | 0.0 | 0.0 |
| 84 | 86.3 | 0.0 | 0.5 | 4.0 | 29.4 | 0.0 | 98.6 | 175.3 | 117.2 | 0.4 |
| Avg | 28.1 | 54.1 | 22.3 | 28.8 | 48.6 | 15.6 | 32.1 | 15.5 | 20.8 | 44.9 |