TABLE 2.
Primers used in this study
| Primer | Sequence (5′ to 3′) |
|---|---|
| gadDF1 | GGCAGATCGCTTACAAATGAAAGGNTGGCARGT |
| gadDF2 | GCGAGACAAAATCTAGCTACGTTYTGYCARAC |
| gadTF1 | AAAGCCGGAAGTGGCAAATGTNGGRTAYTCRTA |
| gadTF2 | CACTTTAACAATTTTGCCACCNGGDATYTGRTA |
| TribestF | ACTTTTATAGCTGGTCATTCCATGGGNGGNTA |
| gadT1SOEA | ACTTCTAGATTTTCACTGGTATTC (XbaI)a |
| gadT1SOEB | CGTCAGTCCCCAGAAAAT |
| gadT1SOEC | ATTTTCTGGGGACTGACGGCTTCCGTTGTGCCATCCAb |
| gadT1SOED | ACCCCCGGGGCCGAATGTCAATAC (XmaI)a |
| gadD3SOEA | GATCTAGAAAATCGGTGCGTC (XbaI)a |
| gadT3SOEB | CCCAATTGTATACTCATC |
| gadD3SOEC | GATGAGTATACAATTGGGACGCACTAAAAAAGAAGAb |
| gadD3SOED | GAGAATTCCAGTACGCGAGCC (EcoRI)a |
| gtcA For | TCCGTGGTACACAGATCA |
| gtcA Rev | GCAATACGATAATCCCAG |
| gadD1SOEA | AATCTAGATACGTTCTGTC |
| gadD1SOEB | CCAACAAACTTGATAGCC |
| gadD1probe1 | TTCTGTGTGTACTGGGAT |
| gadD1probe2 | GTGCCCTGATGTATTAATCG |
a
Base changes made to incorporate cut site (change in boldface type). The relevant restriction enzyme is in parentheses.
b
Overhang complementary to the corresponding SOEB primer is underlined.