TABLE 3.
Growth of L. rhamnosus GG in MRS medium containing different sugars (indicated by OD600 and pH) and survival of L. rhamnosus GG grown in MRS medium containing glucose and exposed to simulated gastric juice, pH 2.0, in the presence of different sugars for 45 mina
| Carbohydrate | OD600b | pH of 16-h culturec | Concn (log10 CFU ml−1) at:
|
|
|---|---|---|---|---|
| Zero timed | 45 min | |||
| Glucose | 2.18 (0.03)e | 3.68 (0.13) | 9.15 (0.03) | 8.91 (0.26) |
| Fructose | 1.808 (0.01) | 3.54 (0.02) | 9.06 (0.08) | 8.86 (0.09) |
| Lactose | 0.599 (0.07) | 5.84 (0.01) | 9.07 (0.04) | 0.99 (0.74) |
| Sucrose | 0.764 (0.08) | 5.53 (0.01) | 9.16 (0.03) | 0.93 (0.63) |
a
The data are the means for triplicate experiments.
b
The optical density at 600 nm (OD600) was recorded following 16 h of growth at 37°C in the different sugars.
c
The medium pH was 6.2 ± 0.2 at the start of growth.
d
Numbers of viable cells.
e
The values in parentheses are the standard errors.