. 2005 Jun;71(6):2970–2978. doi: 10.1128/AEM.71.6.2970-2978.2005

TABLE 1.

Bacterial strains and plasmids used in the study

Strain or plasmid	Relevant phenotype^a	Description	Reference
pCRCluA	Amp^r	CluA cloned in pCR2.1	This study
pMSP3535	Em^r	Nisin-inducible expression vector	5
p3535CluA	Em^r CluA⁺	pMSP 3535 with cloned cluA, nisin inducible	This study
pAMβ1	Em^r	Broad-host-range drug-resistant plasmid from E. faecalis	7
L. lactis ML3	Lac⁺ Clu⁻ Str^s Em^s	Parental Lac⁺ donor	24
L. lactis MG1363	Lac⁻ Clu⁻	Plasmid-cured derivative of 712	12
L. lactis LM2301	Lac⁻ Str^r Em^s	Plasmid-cured, Str^r derivative of L. lactis strain C2	29
L. lactis LM2302	Lac⁻ Str^r Em^r	Plasmid-cured, Em^r derivative of LM2301	41
L. lactis HW002	Lac⁺ Clu⁺ Str^r Em^s	Clumping transconjugant from ML3 × LM2301	This study
L. lactis HL2301A	Lac⁻ Str^r Em^r	LM2301 transformed with p3535CluA	This study
L. lactis HL2301V	Lac⁻ Str^r Em^r	LM2301 transformed with pMSP3535	This study
L. lactis HL3A	Lac⁺ Str^s Em^r	ML3 transformed with p3535 CluA	This study
L. lactis HL3V	Lac⁺ Str^r Em^r	ML3 transformed with pMSP3535	This study
L. lactis HL01Te	Lac⁻ Str^r Em^s Te^r	Spontaneous 2301 mutant, tetracycline resistant	This study
L. lactis JK2301β	Lac⁻ Str^r Em^r	Transformant of LM2301 received pAMβ1	23
L. lactis HW401	Lac⁺ Str^r Em^r	Clumping transconjugant from HW002 × JK2301β	This study
L. lactis HL421	Lac⁺ Str^r Te^r	Clumping transconjugant from HW401 × HL01Te	This study
L. lactis HL221	Lac⁺ Str^r Em^r	Clumping transconjugant from HW002 × LM2302	This study
L. lactis HL222	Lac⁺ Str^r Te^r	Clumping transconjugant from HL221 × HL01Te	This study
L. lactis HL331	Lac⁺ Str^r Te^r	Clumping transconjugant from HW002 × HL01Te	This study
L. lactis HL332	Lac⁺ Str^r Em^r	Clumping transconjugant from HL331 × LM2302	This study

Clu⁺, ability to self-aggregate; Amp^r, ampicillin resistant; Str^r, streptomycin resistant; Em^r, erythromycin resistant; Te^r, tetracycline resistant; Lac⁺, ability to ferment lactose.