TABLE 2.

Lactococcus lactis conjugal mating^a

Set	Donor	Recipient	Nisin induction	Conjugation frequencyb	Comment
A	ML3	LM2301		1.5 × 10−8 ± 6 × 10−9	Original setting
B	HW002	LM2302		2.5 × 10−2 ± 7 × 10−3	“Super donor I”
C	ML3	HL2301A	Yes	7.2 × 10−8 ± 2.9 × 10−8	CluA induced in recipient
D	ML3	HL2301A	No	2.1 × 10−8 ± 1.9 × 10−8	Control without CluA induction
E	ML3	HL2301V	Yes	2.6 × 10−8 ± 1.8 × 10−8	Control with cloning vector only
F	HL3A	LM2301	Yes	3.3 × 10−7 ± 2.0 × 10−7	CluA induced in donor
G	HL3A	LM2301	No	4.3 × 10−8 ± 2.2 × 10−8	Control without CluA induction
H	HL3V	LM2301	Yes	2.7 × 10−8 ± 1.1 × 10−8	Control with cloning vector only

^aAll strains are derivatives of ML3 and C2, and therefore all contain the Agg factor.

^bValues for conjugation frequency are means of three replicates ± standard deviations.