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. 2005 Jun;71(6):3085–3092. doi: 10.1128/AEM.71.6.3085-3092.2005

FIG. 1.

FIG. 1.

Scheme of different constructs for the secretion of XlnB1 and XlnB2 through either the Sec- or the Tat-dependent secretion system. For the Sec-targeted signal sequence, the celA signal sequence (sp celA) plus a 57-nt upstream sequence next to the ATG codon was fused to xlnB1 (pIAF907-SB1). For the Tat-targeted signal sequence, the xlnC signal sequence deleted from the 5′ end (sp xlnC-40), to which the 57 nt upstream of the celA signal sequence were added, was fused to xlnB2 (pIAF907-TB2). PxlnA, xlnA promoter; SD, Shine-Dalgarno sequence; dark box, sequence complementary to the 16S rRNA; ATG, translation initiation codon. The xlnB1 and xlnB2 genes were arranged either in bicistronic operon (pIAF907-SB1TB2) under the control of the xlnA promoter or separately, each preceded by the xlnA promoter (pIAF907-SB1pATB2).