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Western blot analysis of extracellular xylanase produced by S. lividans transformants. Samples of culture supernatant (10 μl) after 72 h of cultivation were separated by SDS-PAGE and then transferred onto nitrocellulose membrane and probed with anti-XlnB1 antibodies coupled with alkaline phosphatase-labeled goat anti-rabbit immunoglobulin. Lane 1, control XlnB1; lane 2, control XlnB2′; lane 3, IAF907-SB1; lane 4, IAF907-TB2; lane 5, IAF907-SB1TB2.
