TABLE 1.
Plasmids used in this study
| Plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| S. lividans | ||
| pIJ702 | Vector containing tsr and mel markers | 11 |
| pIAF42 | pIJ702 derivative containing xlnB1 from S. lividans | 17 |
| pIAF906 | pIJ702 derivative containing xlnA from S. lividans as a 2-kb SphI-SacI fragment where a HindIII and KpnI restriction site were added on each side of the signal sequence | 21 |
| pIAF907 | pIAF906 derivative in which the KpnI, PstI, SphI, and BglII sites were removed and the xlnA coding sequence was eliminated and replaced by a cloning cassette of HindIII, EcoRI, XbaI, and SacI | 21 |
| pIAF911A.8 | pIAF906 derivative in which the xlnA signal sequence was replaced by the celA signal sequence plus 57 nt upstream of it, containing a sequence complementary to the 16S rRNA and two translation initiation codons | 15 |
| pIAF916C | pIAF906 derivative containing the xlnC signal sequence and the xlnC coding sequence | 9 |
| pIAF916C-40 | pIAF916C derivative in which the xlnC signal sequence was deleted of 30 nt from its 5′ end | 9 |
| pIAF907-SB1 | pIAF911A.8 derivative in which the xlnA coding sequence was replaced by the xlnB1 coding sequence | This work |
| pIAF907-TB2 | pIAF916C-40 derivative in which the xlnC coding sequence was replaced by the xlnB2 coding sequence and the 57 nt upstream of the celA signal sequence was added in front of the truncated xlnC signalsequence | This work |
| pIAF907-SB1 TB2 | pIAF907-SB1 derivative in which a fragment containing the signal sequence and xlnB2 present in pIAF907-TB2 was inserted downstream of xlnB1 | This work |
| pIAF907-SB1 pATB2 | pIAF907-SB1 derivative in which the fragment containing the xlnA promoter, the signal sequence, and xlnB2 present in pIAF907-TB2 was inserted downstream of xlnB1 | This work |
| pIAF907-SB1pASB1 | pIAF907-SB1 derivative in which the fragment containing the xlnA promoter, the signal sequence, and xlnB1 present in pIAF907-SB1 was inserted downstream of xlnB1 | This work |
| pIAF907-TB2pATB2 | pIAF907-TB2 derivative in which the fragment containing the xlnA promoter, the signal sequence, and xlnB2 present in pIAF907-TB2 was inserted downstream of xlnB2 | This work |
| pIAF915 | pIAF906 derivative containing the xlnB1 signal sequence | 21 |
| pIAF915B1 | pIAF915 derivative in which the xlnA coding sequence was replaced by the xlnB1 coding sequence | This work |
| pIAF915B2 | pIAF915 derivative in which the xlnA coding sequence was replaced by the xlnB2 coding sequence | This work |
| pIAF916 | pIAF906 derivative containing the xlnC signal sequence | 21 |
| pIAF916B1 | pIAF916 derivative in which the xlnA coding sequence was replaced by the xlnB1 coding sequence | This work |
| pIAF916B2 | pIAF916 derivative in which the xlnA coding sequence was replaced by the xlnB2 coding sequence | This work |
| E. coli | ||
| pTZ19 | Phagemid carrying bla and part of lacZ | Pharmacia |
| pIAF807 | pTZ19 derivative containing promotorless xlnA with a HindIII and KpnI restriction site on each side of the signal sequence | 21 |
| pIAF811-A.8 | pIAF807 derivative in which the xlnA signal sequence was replaced by the celA signal sequence plus 57 nt upstream of it, containing a sequence complementary to the 16S rRNA and two initiation codons | 15 |
| pIAF815 | pIAF807 derivative in which the xlnA signal sequence was replaced by the xlnB1 signal sequence | 21 |
| pIAF815B1 | pIAF815 derivative in which the xlnA coding sequence was replaced by the xlnB1 coding sequence | This work |
| pIAF815B2 | pIAF815 derivative in which the xlnA coding sequence was replaced by the xlnB2 coding sequence | This work |
| pIAF816 | pIAF807 derivative in which the xlnA signal sequence was replaced by the xlnC signal sequence | 21 |
| pIAF816B1 | pIAF816 derivative in which the xlnA coding sequence was replaced by the xlnB1 coding sequence | This work |
| pIAF816B2 | pIAF816 derivative in which the xlnA coding sequence was replaced by the xlnB2 coding sequence | This work |