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Anti-phOx antibody displayed on the B. thuringiensis spore surface can bind antigen. After being washed and blocked with BSA, spores that carry anti-phOx antibody were incubated with either BSA-FITC conjugate or BSA-FITC-phOx (A). Fluorescence (top) and visible light (VIS) (bottom) images are shown for the resulting spores. In the presence of excess phOx, the binding of FITC-BSA-phOx to spores was abolished (B).
