TABLE 1.
Composition of the media used in the experiments
| Medium | Base | Supplement |
|---|---|---|
| Campy-Cefex | Brucella agara (43 g/liter), ferrous sulfateb (0.5 g/liter), sodium bisulfiteb (0.2 g/liter), sodium pyruvateb (0.5 g/liter), deionized water (1 liter) | La ked horse bloodc (50 ml), cefoperazoneb (33 mg), cycloheximideb (0.2 g) |
| mCampy-Cefex | Brucella agar (43 g/liter), ferrous sulfate (0.5 g/liter), sodium bisulfite (0.2 g/liter), sodium pyruvate (0.5 g/liter), deionized water (1 liter) | Lysed horse bloodd (50 ml), cefoperazone (33 mg), amphotericin Bb (2 mg) |
| CAMPY | Brucella agar (43 g/liter), deionized water (1 liter) | Lysed horse blood (70 ml), polymyxin B sulfatee (1 mg), trimethoprimb (0.01 g), vancomycinb (0.01 g), amphotericin B (2 mg), novobiocinb (0.05 g), sodium pyruvate (0.25 g), sodium metabisulfite (0.25 g), ferrous sulfate (0.25 g) |
| mCCDAf | Nutrient broth no. 2 (25 g/liter), bacteriological charcoal (4 g/liter), casein hydrolysate (3 g/liter), sodium desoxycholate (1 g/liter), ferrous sulfate (0.25 g/liter), sodium pyruvate (0.25 g/liter), agar (12 g/liter), deionized water (1 liter) | Cefoperazone (32 mg), amphotericin B (10 mg) |
| Karmalig | Columbia agar base (39 g/liter), activated charcoal (4 g/liter), hemin (0.032 g/liter), deionized water (1 liter) | Sodium pyruvate (100 mg), cefoperazone (32 mg), vancomycin (20 mg), cycloheximide (100 mg) |
| Campy-Line | Brucella agar (43 g/liter), ferrous sulfate (0.5 g/liter), sodium bisulfite (0.2 g/liter), sodium pyruvate (0.5 g/liter), alpha-ketoglutaric acidb (1 g/liter), sodium carbonateb (0.6 g/liter), deionized water (1 liter) | Heminb (10 mg), polymyxin B sulfate (0.35 mg), trimethoprim (5 mg), vancomycin (10 mg), cycloheximide (100 mg), cefoperazone (33 mg), triphenyltetrazolium chlorideb (200 mg) |
a
Acumedia, Lansing, MI.
b
Sigma-Aldrich, St. Louis, MO.
c
Oxoid, Inc., New York, NY.
d
College of Veterinary Medicine, Auburn University.
e
Alexis Corporation, Lausen, Switzerland.
f
Campylobacter selective blood-free agar (CM0739) and CCDA selective supplement (SR0155, Oxoid).
g
Campylobacter agar base (CM0935) and Campylobacter selective supplement (SR0167, Oxoid).