TABLE 1.

Inhibition of adhesion and invasion of various AIEC strains by probiotic L. casei DN-114 001^c

AIEC strain	Coincubation of L. casei DN-114 001 with:
	10% L. casei spent culture SN		10% L. casei neutralized spent culture SN
	% Inhibition of cell associationa	% Inhibition of cell invasionb	% Inhibition of cell associationa	% Inhibition of cell invasionb
LF 82	89.1 ± 2.8	99.7 ± 0.2	84.9 ± 4.1	99.4 ± 0.2
LF 9	87.1 ± 2.4	97.6 ± 0.7	80.3 ± 5.1	97.4 ± 0.7
LF 15	87.4 ± 2.8	98.0 ± 1.0	81.4 ± 3.6	97.8 ± 1.0
LF 31	90.0 ± 3.3	98.9 ± 0.6	86.9 ± 2.9	98.5 ± 0.6
LF 65	87.2 ± 3.4	98.9 ± 0.7	78.6 ± 3.9	98.6 ± 0.7
LF 110	89.1 ± 2.4	99.0 ± 0.6	86.0 ± 2.7	98.4 ± 0.8
LF 134	83.4 ± 2.7	96.1 ± 1.4	76.3 ± 4.0	93.0 ± 2.6

^aInhibition (%) = [(cell-associated bacteria alone − cell-associated bacteria in coincubation experiments with L. casei DN-114 001)/(cell-associated bacteria alone)] × 100.

^bInhibition (%) = [(intracellular bacteria alone − intracellular bacteria in coincubation experiments with L. casei DN-114 001)/(intracellular bacteria alone)] × 100.

^cCoincubation experiments were performed with Intestine-407 cells for 6 h at a multiplicity of infection of 500 for the probiotic strain L. casei DN-114 001 and of 10 for AIEC strains. Results are expressed as a percent inhibition of cell-associated bacteria or intracellular bacteria relative to those obtained in monoinfections with AIEC strains (taken as 100%). Each value is the mean ± SEM of three separate experiments.