TABLE 3.
Tn1546 types found among VRE clinical isolates recovered in environmental samplesa
| Typea | p1p2 (22-1330) | p3p4 (1222-2353) | p5p6 (2227-3525) | p7p8 (2769-4042) | p9p10 (3569-4793) | p11p12 (4675-6353) | p13p14 (6229-8021) | p15p16 (6979-8920) | p17p18 (8889-10473) | p19p1 (10403-10830) |
|---|---|---|---|---|---|---|---|---|---|---|
| PP-3 | (+) | (+) | (+) | (+) | (+) | (+) | (+) | (+) | (−/+) | (−) |
| PP-5 | (+) | (+) | (+) | (+) | (+) | (−) | (+) | (+) | (++)b | (−) |
| PP-16 | (−) | (−) | (−) | (−) | (−) | (+) | (+) | (+) | (+) | (+) |
| PP-17 | (−) | (−) | (−) | (−) | (−) | (+) | (+) | (−) | (+) | (+) |
| X | (−) | (−) | (−) | (−) | (−) | (+) | (+) | (−) | (−) | (+) |
| PP-20 | (−) | (−) | (−) | (−) | (−) | (−) | (+) | (−) | (−) | (+) |
a
Tn1546 types were designed according to Woodford scheme (11). For those that did not have a specific previously described type, we used our own designation (PP from Portugal-Oporto followed by a number randomly chosen). (+), amplification; (−), no amplification; (++), amplification of sequences larger than those of the expected size. For PP-3, amplification was negative for p17p18 but positive using ISEf1-F (5′-GGT GTT ACG ATG TCT GAA ATT GC-3′) and p18.
b
Amplification was positive using ISEf1-F and p18.