FIG. 6.

(A) S. cerevisiae Δcts1 cells expressing KlCTS1 secrete KlCts1p. S. cerevisiae Δcts1 cells harboring either an empty vector (pMW20) or a vector containing KlCTS1 under the control of a galactose-inducible glucose-repressible promoter (pMW20-KlCTS1) were grown in medium containing glucose or galactose overnight at 30°C. Spent culture medium was tested for the presence of KlCts1p by passage over chitin beads, elution of chitin-bound proteins by boiling, separation by SDS-PAGE, and α-ChBD Western blotting. (B) Expression of KlCTS1 corrects the aggregation phenotype of S. cerevisiae Δcts1 cells. S. cerevisiae Δcts1 cells harboring pMW20-KlCTS1 were grown in medium containing galactose or glucose. Cells were fixed in 2.5% glutaraldehyde and visualized by phase-contrast microscopy. Cells harboring an empty vector maintained an aggregation phenotype regardless of the carbon source (data not shown).