TABLE 2.
Phenotypes of various R. capsulatus pyrE and helC mutant strains and their derivatives
| Straina | Plasmida | MedAb
|
MedA + uracilb
|
MedA + uracil + 5FOA (Res)b | Nadid | ||
|---|---|---|---|---|---|---|---|
| Resc | Psc | Res | Ps | ||||
| MT1131 (wild type) | None | + | + | + | + | − | + |
| TJK11 [Δ(pyrE::kan)] | None | − | − | + | + | + | + |
| pRK-PyrE-PB | + | + | + | + | − | NDf | |
| pRK-PyrE-PH | + | + | + | + | − | ND | |
| pRK-PyrESD | − | − | + | + | + | ND | |
| pRK-PyrENde | − | − | + | + | + | ND | |
| pCS1650#3 | + | + | + | + | − | ND | |
| pCS1650#4 | + | + | + | + | − | ND | |
| TCK1 or TCK2 [Δ(pyrE::kan) helC::pyrE]e | None | + | − | + | − | − | − |
| pCS1650#3 | + | − | + | − | ND | − | |
| pCS1650#4 | + | − | + | − | ND | − | |
| pCS1530 | + | + | + | + | ND | + | |
| TCK4 [Δ(pyrE::kan) helC]e | None | − | − | + | − | + | − |
| pCS1650#3 | + | − | + | − | − | − | |
| pCS1650#4 | + | − | + | − | − | − | |
| pCS1530 | − | − | + | + | ND | + | |
| TCK5 [Δ(pyrE::kan) ΔhelC]e | None | − | − | + | − | + | − |
The strains and the plasmids that they carry are described in Table 1.
MedA was the minimal growth medium. MedA + uracil contained 0.1 mg/ml uracil. MedA + uracil + 5FOA contained 0.1 mg/ml uracil and 0.1 mg/ml 5FOA.
Res and Ps indicate respiratory and photosynthetic growth conditions, respectively. +, strain was able to grow; −, strain was not able to grow.
The Nadi reaction was examined for the strains grown on MedA containing uracil under Res conditions. +, Nadi reaction; −, no Nadi reaction.
Strains TCK1 and TCK2 are phenotypically identical, although they differ genotypically. The same is true for TCK4 and TCK5 (for details see Materials and Methods).
ND, not determined.