TABLE 3.
Summary of qcPCR assays
| qcPCR | Primer | Annealing temp (°C) | Length of fragment (bp)
|
Target
|
||
|---|---|---|---|---|---|---|
| Target | Competitor | Phylotype | Phylogenetic group | |||
| BAC | B341f and U533r | 55 | 170-210 | 333 | Most of bacterial phylotypes | Bacteria |
| SOB | B341f and GAM660r | 58 | 319 | 254 | Y56, Y74, Y169, Y182, and Y222 | Thiotrichales |
| SRB | SRB385mf and U926r | 60 | 541 | 437 | δ-Proteobacterial phylotypes | δ-Proteobacteria |
| DSV | B341f and Dv687r | 55 | 346 | 394 | Y29 | Desulfovibrio spp. |
| SVA | Sva1428f and U926r | 55 | 498 | 439 | KY184 | Desulfotalea spp. and relatives |
| DSS | B341f and DSS658r | 58 | 317 | 394 | Y88 and Y183 | Desulfosarcina spp. and relatives |