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. 2000 Dec 1;28(23):e101. doi: 10.1093/nar/28.23.e101

Figure 4.

Figure 4

General expression of novel splice variants of cyclin E in normal and tumor breast cells. RT–PCR amplification of the cyclin E coding sequence from normal and tumor-derived breast epithelial cell lines using oligonucleotides UFCE and CE1247 for EL, C3FOR and CE1247 for Δ48, C31FOR and CE1247 for Δ97 and UFCE and ETREV for ET. The cell lines used were as follows: lane 1, MCF-10A; lane 2, 76N; lane 3, MDA-MB-157; lane 4, MDA-MB-436. Normal cells are represented in lanes 1 and 2 and tumor-derived cell lines in lanes 3 and 4. + indicates a positive control using vectors with the appropriate splice variant cDNA inserts as template. – indicates a negative control using the EL vector as template. The negative control for EL was an empty vector. The first lane of each gel shows the 100 bp ladder molecular weight size markers. Note that the only difference observed between normal and tumor cells was the detection of slower migrating ET primed RT–PCR products which were tumor specific and distinguishable from the ET products based on size.