FIG. 2.

Competition between OAVN and VHOH for OAVN cyclase. Cytosol was incubated in reaction mixtures containing 40 μM OAVN and various concentrations of VHOH, and the amounts of AVR (▪) or VB (▵) produced were then determined by HPLC. The amount of AVR produced (143 pmol/μl) in the reaction mixture without VHOH was defined as 100%. The amount of VB produced (28.5 pmol/μl) in the reaction mixture with 200 μM VHOH was defined as 100%, which was almost same as the amount in the absence of OAVN (data not shown).