TABLE 3.
Ammonia and urea concentration in milk and in cultures of S. thermophilus CNRZ385 after 12 h of growtha
| Samples | Presence of:
|
Concentration (mM)
|
||||
|---|---|---|---|---|---|---|
| Flurofamide | Ammonium chloride | Glutamine | Urea | Ammonia | Nurea+ammoniab | |
| Noninoculated milkc | − | − | − | 3.81 | 0.79 | 8.41 |
| − | + | − | 3.75 | 10.29 | 17.79 | |
| Cultures of S. thermophilus | − | − | − | <0.1* | 6.78* | 6.78* |
| − | + | − | <0.1* | 17.01* | 17.01 | |
| − | − | + | <0.1* | 7.90* | 7.90 | |
| + | − | − | 3.69 | 0.59 | 7.97 | |
| + | + | − | 3.77 | 9.52 | 17.06 | |
| + | − | + | 3.67 | 1.01 | 8.35 | |
a
The values are means of three experiments.
b
Amount of nitrogen present in urea and ammonia.
c
Addition of flurofamide or glutamine did not change ammonia and urea concentration of milk. *, Values after 12 h of growth differed from those of the corresponding noninoculated medium (P < 0.05).