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. 2005 Jun;71(6):3276–3284. doi: 10.1128/AEM.71.6.3276-3284.2005

TABLE 3.

Specific activities of 2-oxo acid decarboxylation by cell extractsa

Nitrogen source Specific decarboxylase activity [nmol · min−1 · (mg protein)−1]
Phenylpyruvate (phenylalanine) α-Ketoisovalerate (valine) α-Ketoisocaproate (leucine) α-Ketomethylvalerate (isoleucine) 3-Methylthio α-ketobutyrate (methionine)
Ammonia BDb BD BD BD BD
Leucine 13.5 ± 0.7 (100) 4 ± 0.01 (29) 6.5 ± 0.7 (48) 4.5 ± 0.7 (33) 5.5 ± 0.6 (41)
Methionine 22.25 ± 1.8 (100) 8.5 ± 0.5 (38) 9.25 ± 0.5 (42) 5.5 ± 0.9 (25) 9 ± 0.01 (40)
Phenylalanine 67.5 ± 0.7 (100) 19 ± 0 (28) 29.5 ± 0.7 (43) 25.7 ± 3.8 (38) 22 ± 0 (32)
Proline BD BD BD BD BD
Asparagine BD BD BD BD BD
a

Prepared from aerobic, glucose-limited chemostat cultures of S. cerevisiae CEN.PK 113-7D grown with different amino acids as the sole nitrogen source. Data are the average ± mean deviation of assays from two independent chemostat cultures. The relative 2-oxo acid activities, expressed as a percentage of phenylpyruvate activity, are in parentheses. The column headings include in parentheses the amino acid which the 2-oxo acid used as a substrate is derived from.

b

BD, below detection limit.

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