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. 2005 Jun;71(6):2902–2909. doi: 10.1128/AEM.71.6.2902-2909.2005

FIG. 1.

FIG. 1.

Organization and cotranscription of the gal operon of A. ferrooxidans. (A) The gal operon consists of luxA-like, galE, galK, pgm, and galM flanked by the genes rbn and mgt. A potential pseudo-tRNA is located between galM and mgt. The direction of transcription is indicated by the block arrows. Locations of PCR primers are shown below the operon. (B) Determination of cotranscription of the genes comprising the gal operon by RT-PCR. Gel electrophoresis of DNA fragments amplified by RT-PCR using purified RNA as a substrate (a) or PCR using genomic DNA as a substrate (b). Arrows indicate the predicted sizes of the amplified DNA fragments in base pairs. (C) A comparison of the organization of the gal operon of A. ferrooxidans with the gal operons of E. coli (18), Lactobacillus casei, and Klebsiella pneumoniae. The organization of these operons was derived from the Integrated Genomics web site (www.integratedgenomics.com).