TABLE 1.
Bacterial strains and plasmids used
| Strain or plasmid | Relevant genotype or phenotypea | Source or reference |
|---|---|---|
| Strains | ||
| A. ferrooxidans | ATCC 23270 (type strain) | American Type Culture Collection |
| Escherichia coli | ||
| DH5α | F− Δ(lacZYA-argF) endA1 hsdR17 (rK mK+) supE44 thi-1 recA1 gyrA96 relA1 φ80lacZ ΔM15 | |
| S491 | F−galT22 his lac proA tonA uraP | National Institute of Genetics; SHIGEN |
| HB101 | F−λ−ara-14 galK2 hsdS20 (r-B,m-B) lacY1 leu mtl-1 proA2 recA13 rpsL20 supE44 thi xyl-5 | 1 |
| CSH41 | F+ Δ(lac-pro) galE thi | National Institute of Genetics; SHIGEN |
| JW1091 | λ−lacZ125 relA1 spoT1 trp-49 zbf-507::Tn10 pgm/zbf-507::Tn10 | National Institute of Genetics; SHIGEN |
| FF4001 | MC4100 galU95 | 21 |
| Plasmids | ||
| PKK223-3 | Ampr | Pharmacia Biotech |
| pUC18 | Ampr | Gibco BRL |
| pgalT-1 | Vector pUC18 with sequence of A. ferrooxidans that expresses GalT activity | This study |
| pgalU-1 | Vector pKK 233-3 with galU of A. ferrooxidans | This study |
| ppgm-1 | Vector pKK 233-3 with pgm of A. ferrooxidans | This study |
| pgalE | Vector pKK 233-3 with galE of A. ferrooxidans | This study |
a
Ampr, ampicillin resistant..