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. 2005 Jun;71(6):2902–2909. doi: 10.1128/AEM.71.6.2902-2909.2005

TABLE 2.

Primers used for PCR experiments and gene cloning and RT-PCR experiments

Gene Positiona Primer used for:
PCR
luxA A 5′ ATGCGGCATTTATTTTGGAGG 3′
B 5′ CGGTGACCAGCACCTGC 3′
galE A 5′ ATGCAGGTGTTGGTCACCGGCG 3′
B 5′ CAAAGTTCATAAGCCATTCTGCCACG 3′
pgm A 5′ ATGGCGGTATTGCAGATTG 3′
B 5′ TCAGGTGATGACAGTGGGG 3′
galU A 5′ ATGGCTGAAGTGCGCAAGGC 3′
B 5′ ACCCACCACCGGACGGGC 3′
RT-PCR
rbn 1 5′ ATGTCCCGAAAGCGATGGT 3′
luxA 2 5′ TAAATGCCGCATCCACCCCG 3′
3 5′ ATGCGGCATTTATTTTGGAGG 3′
galE 4 5′ CGGTGACCAGCACCTGC 3′
5 5′ ATGCAGGTGTTGGTCACCGGCG 3′
galK 6 5′ TCACAAGGCAAAGCCCTGGGTG 3′
7 5′ ATGAACTTTGCTCATCATATTCCTGC 3′
pgm 8 5′ TGCAATACCGCCATGGCA 3′
9 5′ ATGGCGGTATTGCAGATTG 3′
galM 10 5′ GCCAATGGTATCGAGCAGT 3′
11 5′ GACAACGGCATGGGCTAC 3′
mgt 12 5′ CACTTCCGTGGGCTC 3′
a

Position relative to genes shown in Fig. 1A and B.