TABLE 2.
Gene | Positiona | Primer used for: |
---|---|---|
PCR | ||
luxA | A | 5′ ATGCGGCATTTATTTTGGAGG 3′ |
B | 5′ CGGTGACCAGCACCTGC 3′ | |
galE | A | 5′ ATGCAGGTGTTGGTCACCGGCG 3′ |
B | 5′ CAAAGTTCATAAGCCATTCTGCCACG 3′ | |
pgm | A | 5′ ATGGCGGTATTGCAGATTG 3′ |
B | 5′ TCAGGTGATGACAGTGGGG 3′ | |
galU | A | 5′ ATGGCTGAAGTGCGCAAGGC 3′ |
B | 5′ ACCCACCACCGGACGGGC 3′ | |
RT-PCR | ||
rbn | 1 | 5′ ATGTCCCGAAAGCGATGGT 3′ |
luxA | 2 | 5′ TAAATGCCGCATCCACCCCG 3′ |
3 | 5′ ATGCGGCATTTATTTTGGAGG 3′ | |
galE | 4 | 5′ CGGTGACCAGCACCTGC 3′ |
5 | 5′ ATGCAGGTGTTGGTCACCGGCG 3′ | |
galK | 6 | 5′ TCACAAGGCAAAGCCCTGGGTG 3′ |
7 | 5′ ATGAACTTTGCTCATCATATTCCTGC 3′ | |
pgm | 8 | 5′ TGCAATACCGCCATGGCA 3′ |
9 | 5′ ATGGCGGTATTGCAGATTG 3′ | |
galM | 10 | 5′ GCCAATGGTATCGAGCAGT 3′ |
11 | 5′ GACAACGGCATGGGCTAC 3′ | |
mgt | 12 | 5′ CACTTCCGTGGGCTC 3′ |
Position relative to genes shown in Fig. 1A and B.