TABLE 3.

List of candidate genes proposed to be involved in the formation of EPS precursors in A. ferrooxidans and their proposed activities

Genea	Proposed enzyme activityb	Best BlastP hitc	% Simd	Scoree	E valuef	Motif(s)g
galE	UDP-glucose epimerase (EC 5.1.3.2)	Mycobacterium avium	43	93	7e−18	pfam 1370; COG 1087
galK	Galactokinase (EC 2.7.1.6)	Streptomyces coelicor	48	106	5e−22	PD 339735
pgm	Phosphoglucomutase (EC 5.4.2.2)	Synechococcus elongatus	77	711	0.0	pfam 2878; COG 0033
galM	Aldose-1 epimerase (EC 5.1.3.3)	Sinorhizobium meliloti	52	33.9	1.1
galT-like	Possible galactose-1-phosphate urydylyltransferase (EC 2.7.7.10)	Kinecococcus radiotolerans	42	32	4.3	COG 1085
galU	UDP-glucose pyrophosphorylase (EC 2.7.7.9)	Burkholderia fungorum	73	371	2e−73	pfam 0483; COG 0451; PD 01252
luxA-like	Possible coenzyme F420-dependent reductase	Bradyrhizobium japonicum	44	157	3e−37	pfam 0296; COG 2141
rfbA	d-TDP-glucose pyrophosphorylase (EC 2.7.7.24)	Azotobacter vinelandii	80	406	1e−112	pfam 0483; COG 1209
rfbB	d-TDP-glucose4,6dehydratase (EC 4.2.1.46)	Pseudomonas putida	72	443	1e−123	pfam 1370; COG 1088
rfbC	d-TDP-4-dehydrorhamnose 3,5-epimerase (EC 5.1.3.13)	Pseudomona stutzeri	79	245	3e−64	pfam 0908; COG 1898
rfbD	d-TDP-4-dehydrorhamnose reductase (EC 1.1.1.133)	Synechococcus elongatus	63	261	1e−68	pfam 4321; COG 1091

^aProposed gene name.

^bProposed enzyme activity.

^cOrganism with the best BlastP hit to the candidate gene.

^dPercentage of similarity (% Sim) of candidate gene to that found in the organism listed in row (c).

^eScore of BlastP match.

^fE value of BlastP match.

^gMotif and domains identified in the candidate proteins. Pfam, protein families; PD, Prodom (protein domains).