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Physical appearance of smears. Smears were prepared from equal aliquots of a sputum sample processed by the CDC and USP smear methods. (i) A loopful from the purulent portion of sputum was taken in a 3-mm sterile wire loop and smeared onto a glass slide (direct). (ii) Two loopfuls of specimen processed by the CDC method were smeared onto a glass slide. (iii) Ten percent of the USP sputum was smeared onto a glass slide.
