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. 2005 Jun;43(6):2703–2708. doi: 10.1128/JCM.43.6.2703-2708.2005

TABLE 1.

Results of Ziehl-Neelsen staining of sputum smears prepared by USP and direct methods

Smear method and resulta No. of smears Culture resultb (no. of samples)
Positive Negative
USP
    3+ 215 208 7
    2+ 78 75 3
    1+ 33 30 3
    Scantyc 17 9 8
    Any positive 343 322 21
    Negative 228 6 222
    Total 571 328 243
Direct
    3+ 47 46 1
    2+ 70 66 4
    1+ 97 94 3
    Scanty 29 19 10
    Any positive 243 225 18
    Negative 328 103 225
    Total 571 328 243
a

3+, >10 AFB per oil immersion field (OIF) (at least 20 fields); 2+, 2 to 10 AFB per OIF (at least 50 fields); 1+, 1 to 99 AFB in 100 OIF; scanty, 1 to 9 AFB in 100 OIF (40).

b

Using USP, CDC, or modified Petroff method.

c

All were negative by direct smear microscopy.