TABLE 2.
List of archival samples tested with the single-tube TaqMan RT-PCR assay
| Isolate | Country of origin | Host species | Genotype |
CT valuea
|
% Divergenceb
|
||||
|---|---|---|---|---|---|---|---|---|---|
| FAM | HEX | Cy5 | GT1 | GT5 | GT6 | ||||
| 8 | Finland | Human | 6 | 15.86 | 11.1 | 26.9 | 0 | ||
| 9 | Germany | Bat | 5 | 15.73 | 19.8 | 0 | 17.6 | ||
| 11 | Germany | Bat | 5 | 13.94 | 19.8 | 0 | 17.6 | ||
| 19 | Denmark | Bat | 5 | 16.46 | 19.8 | 0 | 17.6 | ||
| 29 | The Netherlands | Bat | 6 | 12.12 | 15.3 | 26.9 | 0 | ||
| 31 | The Netherlands | Bat | 5 | 14.12 | 19.8 | 0 | 17.6 | ||
| 32 | The Netherlands | Bat | 5 | 13.62 | 19.8 | 0 | 17.6 | ||
| 33 | The Netherlands | Bat | 5 | 15.67 | 19.8 | 0 | 17.6 | ||
| 37 | The Netherlands | Bat | 5 | 12.95 | 19.8 | 0 | 17.6 | ||
| 38 | The Netherlands | Bat | 5 | 16.57 | 19.8 | 0 | 17.6 | ||
| 44 | USA | Bat | 1 | 11.72 | 3.6 | 18.9 | 12.0 | ||
| 47 | USA | Bat | 1 | 16.19 | 7.4 | 24.8 | 16.6 | ||
| 48 | USA | Bat | 1 | 44.67 | 11.2 | 24.8 | 21.3 | ||
| 49 | USA | Bat | 1 | 10.95 | 7.2 | 18.9 | 21.3 | ||
| 56 | USA | Fox | 1 | 7.39 | 7.3 | 25.7 | 12.0 | ||
| 57 | USA | Skunk | 1 | 7.91 | 3.6 | 31.5 | 16.6 | ||
| 58 | USA | Skunk | 1 | 12.27 | 7.2 | 37.8 | 12.2 | ||
| 66 | Poland | Bat | 5 | 15.08 | 19.8 | 0 | 17.6 | ||
| 68 | Peru | Dog | 1 | 10.12 | 3.6 | 24.8 | 16.6 | ||
| 73 | Belize | Dog | 1 | 8.95 | 3.6 | 24.8 | 12.0 | ||
| 100 | Morocco | Unknown | 1 | 20.27 | 3.6 | 31.5 | 16.6 | ||
| 114 | Chile | Bat | 1 | 15.36 | 7.4 | 18.9 | 12.0 | ||
| 144 | Germany | Bat | 5 | 29.13 | 19.8 | 0 | 17.6 | ||
| 193 | Pakistan | Canine | 1 | 9.25 | 3.6 | 31.5 | 16.6 | ||
| 202 | Turkey | Canine | 1 | 9.72 | 0 | 24.8 | 12.0 | ||
| 227 | Nigeria | Canine | 1 | 8.2 | 3.6 | 24.8 | 12.0 | ||
| 234 | Russia | Canine | 1 | 13.55 | 0 | 24.8 | 12.0 | ||
| 253 | Russia | Wolf | 1 | 12.31 | 3.5 | 25.7 | 7.8 | ||
| 264 | Ukraine | Bat | 5 | 24.78 | 15.3 | 26.9 | 0 | ||
| 266 | France | Bat | 5 | 8.07 | 24.9 | 0 | 17.6 | ||
| 303 | Russia | Raccoon dog | 1 | 32.49 | 0 | 24.8 | 12.0 | ||
| 305 | Georgia | Dog | 1 | 10.27 | 0 | 24.8 | 12.0 | ||
| 307 | Georgia | Cattle | 1 | 8.15 | 0 | 24.8 | 12.0 | ||
| 313 | Germany | Fox | 1 | 9.08 | 0 | 24.8 | 12.0 | ||
| 318 | Germany | Fox | 1 | 25.89 | 3.5 | 19.4 | 7.8 | ||
| 338 | China | Cattle | 1 | 9.88 | 3.6 | 24.8 | 12.0 | ||
| 341 | China | Dog | 1 | 11.09 | 0 | 24.8 | 12.0 | ||
| 342 | China | Cattle | 1 | 11.27 | 3.6 | 24.8 | 12.0 | ||
| 345 | Germany | Bat | 5 | 23.85 | 19.8 | 0 | 17.6 | ||
| 349 | Germany | Bat | 5 | 24.77 | 19.8 | 0 | 17.6 | ||
| 350 | Germany | Bat | 5 | 24.06 | 19.8 | 0 | 17.6 | ||
| 399 | Botswana | Jackal | 1 | 10.46 | 11.6 | 18.9 | 21.6 | ||
| 437 | Estonia | Raccoon dog | 1 | 12.58 | 0 | 24.8 | 12.0 | ||
| 447 | Botswana | Dog | 1 | 9.8 | 3.6 | 31.5 | 16.6 | ||
| 483 | Botswana | Genet | 1 | 13.55 | 11.6 | 18.9 | 21.6 | ||
| 484 | Botswana | Cattle | 1 | 12.62 | 11.6 | 18.9 | 21.6 | ||
| 498 | S. Africa | Cat | 1 | 8.25 | 3.6 | 24.8 | 16.6 | ||
| 519 | Unknown | Cattle | 1 | 9.29 | 0 | 24.8 | 12.0 | ||
| 594 | Switzerland | Bat | 6 | 16.22 | 15.3 | 26.9 | 0 | ||
| 621 | Switzerland | Bat | 6 | 10.78 | 19.8 | 20.3 | 0 | ||
| 628 | England | Bat | 6 | 12.75 | 15.3 | 26.9 | 0 | ||
| 634 | Australia | Bat | 7 | 24.9 | 31.5 | 33.4 | |||
| 758 | Tanzania | Canine | 1 | 10.06 | 0 | 24.8 | 12 | ||
| 759 | Tanzania | Canine | 1 | 29.35 | 0 | 24.8 | 12 | ||
| 889 | Czech Republic | Fox | 1 | 13.95 | 3.7 | 19.9 | 12.5 | ||
| 960 | Estonia | Raccoon dog | 1 | 13.7 | NA | NA | NA | ||
| 1124 | Turkey | Fox | 1 | 16.93 | NA | NA | NA | ||
| 1125 | Turkey | Fox | 1 | 19.08 | NA | NA | NA | ||
| 1176 | Yugoslavia | Fox | 1 | 13.92 | NA | NA | NA | ||
| 1177 | Yugoslavia | Fox | 1 | 17.65 | 3.6 | 31.5 | 16.6 | ||
| 1312 | Poland | Badger | 1 | 13.79 | 0 | 24.8 | 12.0 | ||
| 1333 | Scotland | Human | 6 | 13.05 | 19.8 | 26.9 | 3.8 | ||
a
For clarity, when no CT value was recorded, this is indicated by the absence of a numerical value for that particular probe.
b
The divergence was calculated by using the Clustal V component of the MegAlign multiple alignment package (DNASTAR Inc.). NA, sequence data are not available for the probe binding region.