TABLE 3.

Strains used in this study and associated multiplex real-time PCR results

Phenotype of strains	Genotype	Total no. of subjects (n = 217j)	No. of positive results with probe specific for:
			O157h	stx2i
E. coli O157:H7a	stx2	30	30	30
E. coli O157:H7	stx1	4	4	0
E. coli O157:H7	Non-stx	1	1	0
Non-O157 clinical E. colib from Taiwan	Othersf	158	0	0
Non-O157 clinical E. colic with stx2 gene from Taiwan	O28 and stx2g	2	0	2
Salmonella Enteritidis strainsd		10	0	0
Shigella spp.e		10	0	0
Pseudomonase		1	0	0
Citrobacter freundii ATCC 8090e		1	0	0

^aAll strains were isolated from the United States (10 strains), Canada (12 strains), Japan (9 strains), and Taiwan (4 strains).

^bAll strains were isolated from clinical samples of diarrhea cases in Taiwan.

^cAll strains were isolated from clinical samples in Taiwan by the Centers for Disease Control, Taiwan.

^dAll strains were isolated from food-borne cases by the Centers for Disease Control, Taiwan.

^eAll strains were from Bioresource Collection and Research Center, Hsinchu, Taiwan.

^fAll genotypes are non-stx₂; and their phenotypes include the following: O1, O3, O6:H?, O6:H12, O8:H?, O8; O14; O15:H21 O15:NM, O18, O20:H20, O23, O25:H51, O25:K98:NM, O26:H?, O26:H11, O27, O28:H?, O28ac:H?, O28ac, O29:H?, O29:NM, O30, O38, O44, O48, O55:H?, O55:H12, O75, O78:H11, O78:H2, O78:K80:H12, O86a, O90, O111ab:H21, O112ac:H?, O114, O115, O123, O124:H?, O125, O127a:H1, O127a:H16, O136:H19, O138, O142:H6, O144, O146:NM, O148, O153:NM, O158, O159:H21, O166, O168, O169:H28, and unknowns.

^gAll genotypes are stx₂, and the serotype was O28ac.

^hNumbers of positive results by the multiplex real-time PCR assay for the rfb_{E. coli O157:H7} gene.

ⁱNumbers of positive results by the multiplex real-time PCR assay for the stx₂ gene.

^jTotal numbers of strains in this study.