TABLE 6.
Sensitivity of the multiplex real-time PCR assay for detection of E. coli O157:H7 strain Tw04 in raw milk and apple juice after enrichment
| No. of CFU/ml | Sensitivity for detection ina:
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Milk
|
Apple juice
|
|||||||||||
| 0 h | 2 h | 3 h | 4 h | 5 h | 10 h | 0 h | 2 h | 3 h | 4 h | 5 h | 10 h | |
| 103 | + | + | + | + | + | + | − | − | + | + | + | + |
| 102 | − | − | + | + | + | + | − | − | + | + | + | + |
| 101 | − | − | − | + | + | + | − | − | − | + | + | + |
| 100 | − | − | − | + | + | + | − | − | − | − | − | + |
| 0 | − | − | − | − | − | − | − | − | − | − | − | − |
a
The plus or minus sign indicates whether or not the relative fluorescent intensity after 40 cycles was greater than the threshold value for the negative control.