Table 3.
Comparison of change folds between oligonucleotide arrays and RT-PCR
| Change fold | |||||||
| Avg Diff | hsotd | hsOtx2 | |||||
| Transcript | hswt | hsotd | hsOtx2 | Array | RT-PCR | Array | RT-PCR |
| scra | 251 | 1375 | 1229 | 5.5 | 1.3 | 4.9 | 1.6 |
| LysD | 525 | 1646 | 2436 | 3.1 | 1.6 | 4.6 | 4.0 |
| glu | 479 | 1196 | 1991 | 2.5 | 1.8 | 4.2 | 10.9 |
| Rpd3 | 1170 | 2562 | 2673 | 2.2 | 2.0 | 2.3 | 2.5 |
| pim | 118 | 246 | 1467 | 2.1 | 1.4 | 12.4 | 8.0 |
| n-syb | 612 | 293 | 296 | -2.1 | -1.5 | -2.1 | -1.5 |
| eyg | 1552 | 229 | 10 | -6.7 | -1.4 | -77.6 | -1.6 |
| wun | 885 | / | 884 | / | / | 1.0 | 1.0 |
| Scc1 | 724 | 723 | / | 1.0 | 1.0 | / | / |
| otd | 84 | 6555 | 108 | 78.0 | 119.4 | 1.3 | 1.5 |
RT-PCR was carried out on cDNA derived from heat-shocked wild type (hswt), heat-shocked otd (hsotd) or heat-shocked Otx2 (hsOtx2) embryos. Change folds determined by RT-PCR are represented as the mean value of eight independent replicates, derived from two different cDNA preparations. wun is used as a control for the comparison of the RT-PCR data between heat-shocked wild type and heat-shocked Otx2. Scc1 is used as a control for the comparison of the RT-PCR data between heat-shocked wild type and heat-shocked otd.