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. 2024 Aug 30;38(11):2355–2365. doi: 10.1038/s41375-024-02391-8

Fig. 4. IL-10R inhibition reverses MM-induced TAM M2 polarization.

Fig. 4

A Effect of anti-IL-10R mAb on patient BM macrophage polarization ex vivo. BM aspirates from 4 MM patients were cultured in the 3DTEBM model, and treated with or without anti-IL-10R mAb (5 µg/ml) for 3 days. Macrophage polarization was tested by flow cytometry and represented as M2/M1 ratio, and a fold-change of non-treated condition (NT). B Effect of anti-IL-10R mAb on macrophage polarization in vitro. THP-1-derived macrophages and primary monocyte-derived macrophages (from 3 independent donors) were co-cultured with MM1S, RPMI8266, or U266 cells (1:1 ratio) and treated with or without anti-IL-10R mAb (5µg/ml) for 3 days. Macrophage polarization was tested by flow cytometry and represented as M2/M1 ratio, and a fold-change of non-treated condition (NT). C Effect of anti-IL-10R mAb on macrophage polarization in a humanized mice model, in vivo. huCD34 NCG mice with human MM tumors were treated with (n = 3) or without (n = 3) anti-IL-10R mAb (5 mg/kg, i.p., twice a week) for 2 weeks. BM was harvested, and macrophage polarization was tested by flow cytometry and represented as M2/M1 ratio, and as fold-change of non-treated condition (NT). (Bars = Average ± SD, *p < 0.05; **p < 0.01; ***p < 0.001;).