TABLE 1.
Nucleotide sequences and positions of primers and probes
| Oligonucleotidea | Sequence | Positionb | Product length (bp) |
|---|---|---|---|
| 16S3 up | 5′-ATGCAAGTCGAGCGAAC-3′ | 28-44 | 282 |
| 16S3 down | 5′-TGTCTCAGTTCCAGTGTGGC-3′ | 309-290 | |
| 16S FITC probe | 5′-CACTTATAGATGGATCCGCGCCGCATTAGCTAGT-FITC-3′ | 199-232 | |
| 16S Red probe | 5′-Red-GTAAGGTAACGGCTTACCAAGGCAACGAT-Phos-3′ | 235-263 |
a
FITC, fluorescein isothiocyanate; Red, LightCycler-Red 640.
b
Position of the nucleotide sequence in the S. epidermidis 16S rRNA gene (accession number L37605).