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Standard curve of the TaqMan HIV-1 RNA real-time PCR test, Abidjan, Côte d'Ivoire (West Africa). The cycle threshold (CT) is the number of cycles before the fluorescence passes a fixed limit (time to positivity). The CT values were obtained from 25 different runs, with three distinct operators. The solid line connects the median values and 25% and 75% interquartile ranges (box plot) of the CT. The vertical lines show the ranges of CT.
