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. 2024 Oct 29;15:9306. doi: 10.1038/s41467-024-53778-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Scheme illustrating a CRISPR-Cas type IV-A locus and its transcriptional interference function. Effector subunits are color-coded according to their identity throughout the manuscript. b Analytical size-exclusion chromatography traces and SDS-PAGE of purified types IV-A1 (left) and IV-A3 (right) effector complexes. ArnA was identified by mass spectrometry as a purification artifact. n = 1. Source data are provided as a Source Data file. c crRNA and DNA substrate sequences of types IV-A1 (top) and IV-A3 (below). Bases resolved in the cryo-EM maps (d, e) are colored orange (crRNA) and blue (DNA). Observed or predicted base pairs are indicated by links between colored or gray bases, respectively. d and e Sharpened experimental cryo-EM maps (top) in two 90°-rotated orientations of the type IV-A1 (d) and type IV-A3 (e) effectors in complex with DNA. Unfiltered experimental and EMReady maps are shown in Supplementary Figs. 2–4. Below: structure models of the type IV-A1 (d) and type IV-A3 (e) effectors in complex with DNA in two 90°-rotated orientations.