Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2024 Oct 29;15:9306. doi: 10.1038/s41467-024-53778-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 6 — a Top: Sharpened experimental cryo-EM map of the type IV-A1 effector in complex with DinG. Unfiltered and EMReady maps are shown in Supplementary Figs. 2, 4. Below: Structure model of the type IV-A1 effector in complex with DinG. b Top: domain organization scheme of DinG. Below: DinG-centered views. DinG domains are color coded according to the scheme above. c Close-up view onto the Cas7-DinG interface in two 180°-rotated orientations. The sharpened experimental cryo-EM map is shown as a translucent surface. d lacZ-CRISPRi assay probing amino acid substitutions in DinG. The scheme on the left illustrates the assay setup. Coloring in the bar graph according to DinG domain coloring in (b). n = 3 independent replicates; mean ± s.d. Residues producing interference defects upon substitution are highlighted in bold in panel (c). Source data are provided as a Source Data file.