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. 2005 Jun;43(6):3023–3026. doi: 10.1128/JCM.43.6.3023-3026.2005

FIG. 1.

FIG. 1.

A. Amplification profile generated by the C. albicans-specific FRET-based probes CA-FLU and LC640-CA in a real-time Lightcycler PCR assay, with F2 channel fluorescence plotted against cycle number showing a sigmoidal increase in F2 fluorescence emission with the C. albicans strain SC5314. Ex-NTC, extraction-no template control; PCR-NTC, PCR-no template control. B. Amplification profile generated by the C. dubliniensis-specific FRET-based probes CD-FLU and LC705-CD in a real-time Lightcycler PCR assay, with F3 channel fluorescence plotted against cycle number demonstrating an increase in F3 fluorescence intensity with the C. dubliniensis type strain CD36 and the original blood culture isolate from the patient.